米氏凯伦藻细胞表面膜蛋白质组及其对温度变化的响应研究     

王坤  王沛  张浩  张树峰  王大志 《海洋与湖沼》2019,50(3):652-663

米氏凯伦藻(Karenia mikimotoi)近年在我国福建、浙江和广东沿海经常形成赤潮,其赤潮不仅影响到海洋生态系统的稳定,也严重威胁到水产养殖以及人类生命健康安全。本论文以米氏凯伦藻为研究对象,建立了米氏凯伦藻细胞表面膜蛋白质荧光标记技术和细胞膜蛋白质提取方法,运用荧光差异凝胶电泳技术(2-DDIGE)对膜蛋白质进行了分析,并研究了米氏凯伦藻的膜蛋白质组及其对环境温度变动的响应。实验共鉴定到44个细胞表面膜蛋白,其中有效注释27个,主要为转运蛋白、HSP70蛋白家族和捕光蛋白等。米氏凯伦藻在20°C条件下的细胞生长和光合作用要明显好于16°C和12°C,但16°C和12°C条件下的差别不大,表明低温限制了米氏凯伦藻的生长。当米氏凯伦藻从12°C快速转移至16°C和20°C时,藻细胞密度和光合作用效率短时间迅速降低,但细胞很快即适应温度变化。细胞膜上的转运蛋白和光合作用蛋白在其适应温度变化中起着重要作用。  相似文献   

延迟交联凝胶研制及其在广西某铀矿堵漏应用          下载免费PDF全文

熊正强  陶士先  刘俊辉  李晓东  薛倩冰 《探矿工程》2020,47(4):140-144

针对地质钻探小井眼条件下失返性漏失堵漏技术难题，开展了适用于地质钻探堵漏要求的聚合物交联凝胶研究。采用单因素优选法，优选了交联凝胶体系用各种处理剂，并以环保型植物胶GJ为成胶剂，加入交联控制剂N1及交联剂1号等组分，研制了一种延迟交联凝胶。该凝胶在广西某铀矿勘查ZK6-37-1孔堵漏成功应用，解决了2个孔段失返性漏失问题。研究结果表明，该凝胶具有交联时间可控(交联时间10～75 min)以及较高的凝胶强度（承压≥6 MPa），在解决失返性漏失方面具有广阔的应用前景。  相似文献   

起伏海面的雷电电磁场传播特征及其对闪电定位精度的影响     

张源源  庞华基  刘钊  宋琳  邓猛 《海洋科学》2020,44(5):34-41

为了研究起伏海面对雷电电磁传播的影响,本文利用Barrick表面阻抗理论和Wait近似算法,采用改进二维分形海面模型模拟起伏海面,利用数值模式,分析起伏海面的雷电电磁传播特征,并进一步讨论了起伏海面对时差法闪电定位系统定位精度的影响。结果表明:起伏海面对垂直电场和磁场的峰值的影响不显著,但会引起波形的上升期时间的延长,浪高越大,影响越明显;随着观测距离的增加,雷电垂直电场波形的上升时间逐渐变长;风速的变化与雷电垂直电场波形的上升时间成正比;由于海面起伏引起雷电电磁场波形在传播中的变化会影响基于时差法闪电定位系统的定位精度,定位误差可达几至十几公里。  相似文献   

蔚县矿区地面沉陷InSAR多维形变监测     

杜建涛  闫丽  赵超英 《煤田地质与勘探》2020,48(1):168-173

河北蔚县是我国华北地区最大的地下采煤区之一，该地区长期存在采矿塌陷灾害，不仅威胁采矿安全，而且严重破坏当地生态环境。本文基于合成孔径雷达干涉（InSAR）这一新型空间对地观测技术，采用61景Sentinel-1A/B干涉宽幅（Interferometric Wide swath，IW）模式数据进行矿区形变观测，获取整个矿区在2017—2018年间的地表形变空间分布特征，并对矿区地表的沉降量级及面积进行详细的统计分析。此外，采用融合多轨道SAR数据的多维形变时序估计方法，对西细庄矿数据进行东西向和垂向的二维形变分解，获取该矿的二维形变时间序列。结果表明:除南留庄井田外，其余三大井田在监测期间均存在不同程度的地面沉陷灾害；整个矿区年沉陷速率超过-10 cm/a的区域达到了2.16 km2；受成像几何影响，不同轨道数据获取的形变结果存在一定差异；西细庄矿以垂向形变为主，伴随明显的东西向水平形变。研究结果为蔚县矿区地面沉陷监测与煤矿安全开采提供数据参考。   相似文献   

改进分数阶达尔文粒子群的多Renyi熵图像分割算法     

袁玉珠 《测绘通报》2019,(6):34-40

针对智能优化图像分割算法易陷入局部最优、分割精度不高等问题，本文融合改进的分数阶达尔文粒子群算法和二维Renyi熵多阈值，提出了一种新的多阈值遥感图像分割算法。算法利用粒子自身进化信息来定义进化因子，结合进化因子并利用高斯图函数调整分数阶次α系数以实现精确计算和快速收敛；根据局部最优概率因子对局部最优位置进行Levy飞行随机扰动以提高算法跳出局部最优的能力；同时将二维Renyi熵单阈值扩展到多阈值分割上，并结合改进的分数阶达尔文粒子群算法，将二维Renyi熵多阈值应用于遥感图像分割中。仿真结果表明，与其他2种智能优化分割算法相比，本文分割算法在细节处理和分割精度上均有明显优势，在PRI上至少提升7.27%、VOI至少降低6.5%、GCE至少降低10.4%。  相似文献   

An improved building detection approach using L-band POLSAR two-dimensional time-frequency decomposition over oriented built-up areas     

Lei Deng  Ying He  Zhi-hui Mao  Jie Yu 《地理信息系统科学与遥感》2019,56(1):1-21

The objective of this study is to efficiently extract detailed information about various man-made targets in oriented built-up areas using polarimetric synthetic aperture radar (POLSAR) images. This paper develops an improved approach for building detection by utilizing Two-Dimensional Time-Frequency (2-D TF) decomposition. This method performs outstandingly in distinguishing between man-made and natural targets based on the isotropic behaviors, frequency-sensitive responses, and scattering mechanisms of objects. The proposed method can preserve the spatial resolution and exploit the advantages of TF decomposition; specifically, the exact outlines of buildings can be effectively located, and more types of features (e.g., flat roofs, roads, and walls that are oblique to the radar illumination) can be distinguished from forests in complex built-up areas by 2-D TF decomposition. The coarser-resolution subaperture images that are produced in the azimuth direction, which correspond to different looking angles, are beneficial for detecting man-made structures with main scattering centers oriented at oblique angles with respect to the radar illumination. In the range direction, the obtained subaperture images, which correspond to various observation frequencies, can be helpful in distinguishing flat roofs and roads from forests. This method was successfully implemented to analyze both NASA/JPL L-band AIRSAR and L-band EMISAR data sets. The building detection results of the proposed method exhibit a significant improvement over those of other methods and reach an overall accuracy over 80%, with approximately 20% higher than the accuracies of K-means clustering and the entropy/alpha-Wishart classifier and approximately 10% higher than the accuracy of the support vector machine method. Moreover, building details can be precisely detected, obliquely oriented buildings can be identified, and the distinction between buildings and forests is significantly improved, as both visually and statistically indicated. This method is highly adaptable and has substantial application value.  相似文献   

The role of hydrothermal fluids in sedimentation in saline alkaline lakes: Evidence from Nasikie Engida,Kenya Rift Valley     

Robin W. Renaut  R. Bernhart Owen  Tim K. Lowenstein  Gijs De Cort  Emma Mcnulty  Jennifer J. Scott  Anthony Mbuthia 《Sedimentology》2021,68(1):108-134

Saline alkaline lakes that precipitate sodium carbonate evaporites are most common in volcanic terrains in semi‐arid environments. Processes that lead to trona precipitation are poorly understood compared to those in sulphate‐dominated and chloride‐dominated lake brines. Nasikie Engida (Little Magadi) in the southern Kenya Rift shows the initial stages of soda evaporite formation. This small shallow (<2 m deep; 7 km long) lake is recharged by alkaline hot springs and seasonal runoff but unlike neighbouring Lake Magadi is perennial. This study aims to understand modern sedimentary and geochemical processes in Nasikie Engida and to assess the importance of geothermal fluids in evaporite formation. Perennial hot‐spring inflow waters along the northern shoreline evaporate and become saturated with respect to nahcolite and trona, which precipitate in the southern part of the lake, up to 6 km from the hot springs. Nahcolite (NaHCO₃) forms bladed crystals that nucleate on the lake floor. Trona (Na₂CO₃·NaHCO₃·2H₂O) precipitates from more concentrated brines as rafts and as bottom‐nucleated shrubs of acicular crystals that coalesce laterally to form bedded trona. Many processes modify the fluid composition as it evolves. Silica is removed as gels and by early diagenetic reactions and diatoms. Sulphate is depleted by bacterial reduction. Potassium and chloride, of moderate concentration, remain conservative in the brine. Clastic sedimentation is relatively minor because of the predominant hydrothermal inflow. Nahcolite precipitates when and where pCO₂ is high, notably near sublacustrine spring discharge. Results from Nasikie Engida show that hot spring discharge has maintained the lake for at least 2 kyr, and that the evaporite formation is strongly influenced by local discharge of carbon dioxide. Brine evolution and evaporite deposition at Nasikie Engida help to explain conditions under which ancient sodium carbonate evaporites formed, including those in other East African rift basins, the Eocene Green River Formation (western USA), and elsewhere.  相似文献   

某GPS数据处理软件功能探讨     

李佳  许张柱 《中国勘察设计》2011,(5):58-61

针对某GPS数据处理软件在使用过程中存在的问题,结合测量基础知识,浅谈认识,提出整改意见。  相似文献   

弱冻胶调剖剂在孤岛油田污水中成胶性能评价     

宋岱锋  张岩  薄纯辉  李楠  陈辉 《海洋地质译丛》2011,(2):62-64,90

应用孤岛油田现场聚合物和美国进口复合有机铬交联剂TIORCOWC684进行了一系列的室内评价实验。实验结果表明,宝莫聚合物均具有较好的初始黏度、且形成的弱冻胶体系强度高;弱冻胶主段塞选取聚合物浓度2500--3000mg／L,交联剂浓度300--600mg／L,成胶强度较高,且胶体不易脱水;弱冻胶封口段塞聚合物浓度为4000mg／L,交联剂浓度300--900mg／L,胶体强度较大、韧性好,满足封口的要求;同时评价了封口段塞的耐盐性能,结果表明该体系随盐类矿化度的增加体系强度有增加趋势。  相似文献   

海洋废弃钻井液处理技术研究     

徐良伟  马超  陈书明  雷宇 《海洋地质译丛》2011,(4):84-86,92

针对滨海-6井废弃钻井液的特点,提出了破胶-固液分离-固化的思想。通过实验优化破胶剂、絮凝剂、固化剂配方。实验结果表明：100mL废弃钻井液破胶剂加量为2g,絮凝剂为2gAS＋2．5％HPAM溶液（质量分数0．1％）,固液分层现象明显、出水水质透明。固化剂的加量为1．5％CJ-1、27．7％水泥、7．7％CJ-2,6．2％CJ-3。固化强度和浸出液试验表明：固化体在养护15天后平均强度3．0MPa,浸出液指标达到GB8978-1996《污水综合排放标准》二级标准。  相似文献